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Cells were washed to remove viral particles and transduced cells were selected in the presence of 1 g/ml puromycin (Sigma-Aldrich, P8833) for at least 96 h

While older treatments (like Ozempic) mimic only one hormone, Mounjaro mimics two: GLP-1 and GIP

Briefly, biological samples were subjected to protein precipitation with organic solvents and then analyzed by reversed-phase ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry, using the operational conditions described elsewhere [23]